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Progeny from putative gain-of-resistance mutants and "sister" M2 plants (a total of six M2 lines per M1) were inoculated with PXO87 to confirm the mutation.
From a pool of four small RNA source libraries constructed in parallel, including virus-infected wild type Arabidopsis (Col-0) as well as the rdr1 and rdr6 mutant plants, and mock-infected wild type plants, a total of 5,203,265 raw sequence tags were generated from a single run of Illumina's sequencing-by-synthesis (SBS) platform (Fig. 1B).
In the control plants, a total of 58 inflorescences were evaluated, which developed only normal flowers.
After further database searching and alignment with known bZIP proteins from other plants, a total of 69 non-redundant SlbZIP TFs were identified.
For flowering time in the non-vernalized plants, a total of 12 QTL were identified in 8 LGs (Supplementary Table S2 and Fig. 1).
For the field grown plants, a total of 150 Li 1, 100 Li 2, and 100 WT plants were grown in a field at the USDA-ARS Southern Regional Research Center, New Orleans, LA in the summer of 2013.
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Plans for Heartwood include planting a total of 600,000 native trees, a community orchard (local schoolchildren helped plant the first fruit trees in 2010), wildflower meadows and miles of footpaths.
The park plans to plant a total of 200 trees over time.
For each plant, a total of ten leaves were wounded, one leaf per day, over a period of ten days.
Each sample consists of pooled tissues from a group of 6 plants (from a total of 18 plants).
One of those projects: A new natural-gas-fired plant in Kentucky that will replace three half-century-old coal plants with a total of 800 megawatts of capacity.
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