Sentence examples for planta material from inspiring English sources
cDNA libraries were constructed from ex planta material (resting and germinating urediniospores, germ tubes, etc). of four different Melampsora taxa.
Total RNA was extracted from ex planta material and purified haustoria using the RNeasy mini kit (Qiagen, Valencia, CA, USA) and the Absolutely RNA® miniprep kit (Stratagene, La Jolla, CA, USA), respectively.
We constructed cDNA libraries from ex planta material of four different Melampsora taxa with different host specificities: the Eurasian M. larici-populina, the North American M. occidentalis, and two formae speciales of the North American M. medusae, M. medusae f. sp. deltoidae and M. medusae f. sp. tremuloidae (Table 1; see Methods).
We developed a read-mapping pipeline to distinguish fungal and host transcripts in the in planta samples (supplementary fig. S1, Supplementary Material online); our final data sets comprised 45.1 50.2 million mapped reads from samples of Z. tritici axenic culture and 4.2 5.3 million Z. tritici reads from samples of infected plant tissue (supplementary table S1, Supplementary Material online).
In keeping with this expectation, after removing T. subtilis and R. contractilis from our multigene alignment, support for the haptophyte/cryptomonad/SAR group and its sister grouping with Plantae both increased substantially (supplementary fig. 3, Supplementary Material online).
In particular, we collected the set of F. graminearum genes that are detected as differentially expressed in planta in at least one of several experiments (see Materials and Methods) and are under diversifying selection, that is, they pass the CODEML LRTs if they have at least two orthologs or they have a d N / d S ratio larger than the genome-wide mean if they only have one ortholog.
Rather than expanding development of current food, bio-energy feedstock and fibre products, some new technologies enhance in planta production of novel compounds that are of interest to the chemicals and materials manufacturing industries (Napier 2007).
No combination of PDZ and LIM domain(s) was found for taxons in the Plantae or Fungi lineages with the BLAST algorithm [4] in the databases used (see Material and Methods).
Using plant products instead of fossil oil as the source for the material and chemicals could capture much of this added value if the plant molecules are optimised in planta for the end use and thereby minimising the downstream processing costs.
Although there is room for improvement (e.g., reducing rAH's aggregate formation in planta), we obtained a comparatively high expression level (up to 120 mg of functional rAH per kg of fresh leaf material) for plant-based expression systems in general [55].
