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Cell wall modification via in planta engineering can be utilized to design cell wall constituents with increased fermentable sugars, polymer extractability, or to tailor other lignocellulose properties [ 1, 2].
The results indicate that the cellulose biosynthetic pathway is tightly regulated, that individual CesA proteins may play different roles in the synthase complex, and that the sensitivity to CesA gene manipulation observed here suggests that in planta engineering of cellulose levels is likely to require more sophisticated strategies.
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Here, we report a novel in planta "exon engineering" strategy for cloning of full-length coding sequence of ABC transporters followed by methods for biochemical characterization of ABC exporters in Xenopus oocytes.
Here we summarize the main achievements in the field of in planta glyco-engineering for the production of therapeutically relevant proteins.
The recent cloning and characterization of β-carotene ketolase genes in conjunction with the development of effective co-transformation strategies permitting facile co-integration of multiple transgenes in target plants provided essential resources and tools to produce ketocarotenoids in planta by genetic engineering.
To sum up, the application of plant laccase in biomass engineering through in planta manipulation of laccase/miRNA promises a bright development prospect, although no practice case has ever been reported in energy plant.
Taken together, our knowledge of the physiological and molecular effects as well as the actual site of action of a particular harpin in planta may have an impact on genetic engineering strategies that aim to increase plant resistance.
Indeed, recent studies have shown that clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Casystemsthat targetargethehe genomes of DNA viruses can interfere with viral activity and limit viral symptoms in planta, demonstrating the utility of this system for engineering molecular immunity in plants.
Here, we describe the engineering of entirely novel metabolic pathways in planta enabling generation of halogenated indigo precursors as non-natural plant products.
Exploiting the wealth of information currently available on in planta oil biosynthesis, and coupling this information with the tool of genetic engineering, it is now feasible to deliberately alter fatty acid biosynthetic pathways to generate unique oils in commodity crops.
Not only genes that are involved in the biosynthesis of secondary metabolites but also genes that are involved in their transport will be important for systematic metabolic engineering aimed at increasing the productivity of valuable secondary metabolites in planta.
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