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Immature pods were harvested and aseptically dissected, and embryos were cultured with carbon and nitrogen sources to mimic in planta development as previously described.
However, the fungal biomass ratio (% of fungal DNA in the infected leaf) in the NIL with qNLB1.02 Tx303 was 24% higher than in B73 (P < 0.0001), indicating that the in planta development of S. turcica is more extensive on plants without qNLB1.02 B73.
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During in planta growth and development of filamentous intracellular hyphae, a close relationship with host cells is formed, enabling the uptake of nutrients, such as carbon and nitrogen by the pathogen, as well as secretion of proteins into host cells [ 18].
Although it remains plausible that paralogous activities of eIF iso 4E may compensate for these dysfunctions in planta, growth defects have been described for an Arabidopsis mutant line lacking eIF4E [51] suggesting that mutations leading to the R/− phenotype may similarly affect pea development.
Assays to detect subtle differences in growth in planta or in disease development are labour-intensive.
These are complicated by the fact that targeted proteases are often important for plant development, the broad spectrum of potential protease targets and compromises resulting from alternative in planta targeting strategies.
We found that the limiting stage for fungal development in planta is the formation of condiophores.
Trypan blue staining and KOH-aniline blue fluorescence microscopy techniques were used for histological examination of NLB development in planta.
The expression of the auxin efflux regulator PIN1, involved in generating auxin maxima in PR and LRs (Petrášek and Friml, 2009), was examined during AR development in planta.
For example, glycosylation must be considered for the expression and production of cellobiohydrolases in planta, or for the development of advanced biocatalysts for consolidated bioprocessing.
We achieved a continuous observation of fungal development in planta by constitutive expression of the dsRed gene from Discosoma spec. The expression of GFP gene driven by the endogenous TRI5 promoter in the reporter strain enabled monitoring of the trichothecene induction during infection in real time.
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