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Taken together, the in planta data validates our homology models.
In vitro data confirming a specific role for MADS domain protein tetramers has recently emerged [ 70, 71], but in planta data is still lacking.
Our new in planta data also show some limitations of interpreting only oocyte data, even though oocytes allow important testing of models, as done in previous studies.
These new findings also point to limitations of the Xenopus oocyte system (rather than the in planta data) and point to the relevance of our new in planta findings.
In addition, our in planta data show the unexpected interdependence of the Ca2+-independent and Ca2+-dependent signaling pathway, contrary to present less-directly derived models, which will be of interest to a broad readership.
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If we apply this proxy to look for patterns of correlated evolution of TAP gene families and complexity on the complete Plantae data set, we find that all but 10 TF and 8 TR families show significantly correlated evolution with complexity (q ≤ 0.05; supplementary table 8, Supplementary Material online).
We showed that combined with in planta expression data, diversifying selection is a powerful signal for predicting pathogenicity genes involved in the arms race between host and pathogen.
The in planta BiFC data substantiated the specific interaction of ARR22 with AHP2, AHP3 and AHP5 and suggest that ARR22 exerts its phospho-histidine phosphatase activity not only on AHP5 [ 30] but probably also on AHP2 and AHP3.
and 3) Can the signal of adaptation combined with in planta expression data be used to predict genes involved in host- Fusarium interactions, such as Avr genes, elicitors, and host-specific toxins employed in the early biotrophic phase?
For the identification of genes expressed in planta, microarrays data for gene induction fold at 2 days post inoculation on sunflower cotyledons and Expressed Sequence Tags (ESTs) from [ 12] were used.
We showed that adaptive evolution generates a two-speed genome structure for F. graminearum, F. verticillioides, and F. oxysporum f. sp. lycopersici, both on the chromosomal and the gene group level, and that the signal of adaptation combined with in planta expression data can be used to identify individual genes involved in Fusarium-host interactions.
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