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Three gene regions (rbcL, matK, ITS2) were amplified using the CCDB plant protocol [ 46, 47] with Platinum ® Taq DNA polymerase (Invitrogen), and pre-made frozen plates [ 48].
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Highly efficient, reproducible plant regeneration protocol has been standardized in cabbage cv.
In this study, an efficient plant regeneration protocol in vitro and transformation by Agrobacterium-mediated method of Camellia sinensis was achieved, which would lay the foundation for genetic improvement of tea plant by genetic engineering technology.
DNA extraction proceeded according to the DNeasy Plant kit protocol.
SK helped design the plant materials protocol, maintained and harvested plants, and isolated RNA.
Total genomic DNA was extracted using the DNeasy Plant MiniKit protocol (Qiagen, Hilden, Germany).
Plant regeneration protocol of treated samples was the same as described above.
Whole genomic DNA was extracted from leaf tissue using the DNeasy Plant Mini protocol (Qiagen, Hilden).
The plant regeneration protocol of 3,4-DHP-treated samples was the same as described above.
Amplified fragment length polymorphism analysis was performed according to the AFLP plant mapping protocol of Applied Biosystems , Inc
Seed germination and growth followed an arid western plant growth protocol previously used for Se hyperaccumulating plants and described by Sors et al. [ 35].
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