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Glycine is a product of the unique plant pathway of photorespiration (see below Photosynthesis).
We also reconstitute a 14-step biosynthetic pathway of noscapine from the simple alkaloid norlaudanosoline by engineering a yeast strain expressing 16 heterologous plant enzymes, achieving reconstitution of a complex plant pathway in a microbial host.
Together with new genome mining algorithms for plant pathway discovery and advances in plant synthetic biology, this advance provides new routes to synthesize and access previously inaccessible natural products and analogs and has the potential to reinvigorate drug discovery pipelines.
Analysis of the genome indicates that the C. reinhardtii sterol biosynthetic pathway from squalene epoxide to episterol most closely follows the higher plant pathway.
Analysis of the C. reinhardtii genome sequence reveals that this algae is also likely to synthesize sterols using a pathway resembling the higher plant pathway, indicating that its sterols are synthesized somewhat differently than in fungi.
The plant pathway (shown in fig 1) was chosen for L-AA production in yeast cells for two reasons: First of all, GDP-mannose, a key intermediate in this pathway, is naturally produced in yeasts for cell wall construction [14].
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For overproduction and isolation of high-value plant-derived chemicals, plant pathways can be reconstituted in heterologous hosts.
As plant pathways are elucidated, and can be heterologously expressed in hosts that are more amenable to genetic manipulation, biosynthetic production of new-to-nature compounds from plant pathways will become more widespread.
The past decade has seen some impressive successes in the metabolic engineering of biotechnologically important plant pathways.
Alternatively, plant pathways can be modified in the native producer to confer new properties to the plant, such as better biofuel production or enhanced nutritional value.
Selected studies demonstrating the prospects of these approaches, or combinations of them, for understanding the control of flux through particular plant pathways are discussed.
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