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A voucher was made from a plant from this population and deposited in TEX.
A single F1 plant from this cross was self-pollinated, and 168 F2 plants were obtained.
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The specific epithet means "unlettered" or "unmarked" and was intended to distinguish this plant from the classical hyacinth of Greek mythology.
Lesion length of individual T2 plants from this experiment are shown in Additional file 4: Figure S4.
There are many famous medicinal plants from this family, such as Andrographis paniculata, Baphicacanthus cusia, and Dicliptera chinensis.
Thus, plants from this variant had light green color and subnormal turgor of leave cells and the highest leaf growth, but low increase in root number.
Plants from this genus are renowned for their biological activities such as antibacterial, antioxidant, antitumor, antidiabetic, antimicrobial, anxiolytic, sedative and anti-inflammatory activities (Rajabia et al. 2014).
The B line has viable pollen grains and normal seed setting, so it can pollinate the A line, while the F1 plants from this pollination are male sterile, again.
However, all F1 plants from this cross were sterile.
Plants from this generation were haphazardly assigned to act as pollen recipients (target plants) or pollen donors.
Though all plants from this population were self incompatible, we managed by repeated selfing to obtain a few seeds from one highly embryogenic (E) plant, K59.
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