This was an exploratory retrospective genetic study, with planned plasma sample collection from patients with castration-resistant advanced prostate cancer.
Planning for sample collection.
SGA is the Principle Investigator and grant holder and was involved in planning, coordinating the research, sample collection and handling, supervising data entry and analysis, writing and editing of the manuscript.
MJSH is the principle investigator and was involved in planning, coordinating the research, sample collection, handling, writing and editing of the manuscript, SA was involved in coordinating and supervising data entry and analysis and was involved in planning, coordination of the research at the Hospital family planning center, and reviewing of the paper.
BG was involved in the experimental design and in planning the study, carried out sample collection, extracted RNA, carried out microarray molecular work, RT-PCR and real-time PCR work, performed statistical analyses, interpreted data and drafted the paper.
Because each participating city is unique, each had its own sample collection plan, which varied depending on population, location, number of participating collectors and other variables.
A new sample collection plan.
Serial post-treatment blood sample collection was also planned before the 2nd, 3rd, 4th, and 5th cycle of chemotherapy and at disease progression for each patient.
When collecting soil samples from Blake's sorghum field we found ourselves changing the method of sample collection that was originally planned.
To get these high response rates, we have used the venous punctures that were already planned in routine care for additional blood sample collection when this was possible.
