We described herein the molecular design of novel in vivo anti-inflammatory 6-methanesulfonamide-3,4-methylenedioxyphenyl-N-acylhydrazone derivatives (1) planned by applying the molecular hybridization approach.
These compounds were planned by applying the molecular hybridization strategy to propose the structural modifications on the previously described functionalized 2-methyl-imidazolyl-3-acylhydrazone class (2), which presented an important analgesic profile.
To this end, we planned the construction of a Suppression Subtractive Hybridization (SSH) library to identify genes involved in panoistic choriogenesis, using the cockroach Blattella germanica as model.
UD planned and performed culturing fibroblasts, RNA-works, microarray hybridizations, microarray data analysis and management (data set 2 and 3), and contributed essentially to writing the manuscript.
Based on an established platform, linear RNA-amplification, labelling and hybridization on human genome wide oligo-arrays (transcriptome analysis) are planned.
ML participated in planning the microarray experiments, microarray hybridization, and carried out data analysis.
Dye-swap was included in the hybridization plan.
Hybridization plan was as follows: WTCy3 vs. pTrc99ACy5, WTCy5 vs. pTrc99ACy3, WTCy3 vs. pTrc -LacI Cy5, WTCy5 vs. pTrc -LacI Cy5.
MCB was involved in designing and planning the study, collecting the tissues, in oligoarray hybridization, data analysis and in the writing of the manuscript.
This hybridization, between centralized and distributed coordination, can favor the interleaving of planning and execution, some part of global plan can be repaired when others are in execution.
