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These beams have a ring-shaped cross section at the plane of detection.
Every phase distribution results in a different speckle pattern at the region of interest located at the focal plane of detection objective 1. Snapshots of camera images of these speckle patterns for each population member are then evaluated.
As the relative curvatures between the wavefronts change during the propagation, it can be seen that the OPDs at the plane of detection also depend on the actual position of the detector.
The capillary was then set 1 mm behind the tape into the focal plane of detection objective 1. Figure 5(a) shows exemplary the speckle patterns before and after optimization as well as the corresponding fluorescence excitation recorded with both detection objectives.
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Since the surface of the detected object is not on the plane of the detection platform, the stripes from the lamp house will produce a deformed line on the across the detected object.
Custom-made optical fiber bundles enable illumination of the region of interest in the plane of acoustic detection.
Fig. 7 Averaged line plots (black lines) and their standard deviation (shaded area with errorbars) over the light sheets in the middle of the ROI at the focal plane of the detection objective 1 (a) for optimizations behind scotch tape (b) for optimizations inside the glass capillary behind scotch tape and (c) for optimizations behind living drosophila pupae.
The focal plane of the detection objective was imaged onto an sCMOS camera (Zyla, Andor).
Light sheets were generated with cylindrical lenses (f=50 mm) and projected with telescopes and the illumination objectives onto the focal plane of the detection lens.
The pair of alignment lasers ensured that the animals were positioned reproducibly with an accuracy (height) of about ±0.5 mm at the focal plane of the detection system, so that the complete system response was consistent.
Due to inadequate resolution and sensitivity of 18F-FDG PET/CT imaging (∼5 mm in plane) for detection of small size (∼2 3 mm) early pancreatic carcinoma lesions [5], we have focused on the development of imaging agents for visualization of tumor-induced biomarkers that are over-expressed in the peritumoral pancreratic tissue.
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