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After the experimental studies, 350 lux value targeted in the work plane is achieved with ±10 lux error.
Here, we report on the implementation of FMM in which laser intensity modulation at the focal plane is achieved using acousto-optic modulators (AOM).
The arrangement of the raptorial appendages in mantis shrimps, near parallel to the axial plane, is achieved by the presence of an additional joint, resulting in a z-shape.
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Alignment of 5° to 10° of valgus in the frontal plane and within 2° of neutral in the sagittal plane was achieved consistently.
Alignment for an insert within 1 mrad in a vertical plane was achieved by an ex-situ adjustment at installation work.
These results indicated that 1-nm-deep mechanical processing from the surface of one cleavage plane to that of the next periodic cleavage plane was achieved by sliding the tip several times at a slightly larger load than the critical load at which the processing was started.
Delineation of the cell boundary on each plane was achieved by applying two sequential filtering steps, a two-dimensional, nearest-neighbor deconvolution step to increase the signal-to-noise ratio of the boundary while at the same time evening out the local fluctuations in fluorescence signal, followed by a smoothening Gaussian 2-dimensional radial transformation (radius = 1 pixel).
Laser beam scanning in the x-y plane was achieved by a pair of high-speed galvanometer mirrors (Cambridge Technology).
Uniform resolution enhancement along the lateral plane was achieved by placing into the switching beam path a phase plate forming a donut-shaped intensity distribution in the focal plane.
Male Wistar rats (300 g to 350 g) were anaesthetized with sodium pentobarbital (60 mg/kg i.p .. Once surgical plane was achieved, hearts were rapidly excised and rinsed by immersion in ice-cold Krebs-Henseleit buffer, followed by cannulation of the aorta and beginning of the retrograde perfusion at constant flow (10 mL/min).
Whereas the improvement to scanning of the focal plane was achieved by changing the angle of the collimated light entering the back of the objective, the key to adjusting the position of the axial focus is to change the divergence of the beam at the back of the microscope objective.
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