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From each subject, approximately 3 ml of blood was collected aseptically by trained health personnel for genomic DNA extraction (1 ml anti-coagulated blood) and viral serology (2 ml blood in sterile plain vial).
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Initially, 2 mL of blood sample was collected from each CHC patient in plain vials and serum was separated within 2 hrs of blood collection by centrifugation at 2,000 rpm for 10 min at 4°C.
Then, transfer the homogenate into a clean plain glass wide-neck vial (sample vial), using a glass Pasteur pipette.
Transfer the defrosted liquid sample into a clean plain glass wide-neck vial (sample vial) while carefully measuring and recording the volume.
When dealing with "tough" tissues (e.g., muscle, skin sections) place the frozen tissue directly into a clean plain glass wide-neck vial (sample vial) containing 4 ml of ice-cold 15% methanol (v/v) in water.
If required, remove a suitable aliquot for protein content determination and transfer the liquid into a clean plain glass wide-neck vial (sample vial) while carefully measuring and recording the volume.
Blood samples were collected within a week after obtaining lateral cephalometric radiographs from the subjects for analysis of IGF-1 and IGFBP-3 by using red plain blood collection vials (Vacutech).
Five milliliters of blood was collected from median cubital vein by venipuncture in red plain blood collection vials for each subject between 0900 and 1000 hours to avoid diurnal variations, and the timings were matched with taking of lateral cephalograms.
Serum was generated by centrifugation of blood samples left to coagulate for no longer than 1 h at 22°C in plain, endotoxin free vials.
Blood samples drawn from the orbital sinus of the rats before killing were collected in plain and heparin vials for measuring the bilirubin level and liver function enzyme activity as well as O2− and superoxide dismutase (SOD).
Transfer the clear supernatant to a clean plain glass wide-neck vial.
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