Among the 80 genes placed on the array, 30 genes showed one or more clear hybridization peaks.
In preparation for normalization, probes were removed from the datasets if they were one of the negative or positive controls placed on the array by the manufacturer.
No limits are placed on the array dimensions in this library, so the number of atoms that can be displayed is technically only limited by available memory.
The study shows that, due to the restrictions placed on the array location, the likely power production that can be achieved is considerably less than what an analytical prediction suggests might be obtained from the channel.
The resolution of array CGH is determined by the size and number of the clones placed on the array to interrogate genome copy number changes.
In selecting the genes to be placed on the array, our major rationale was to examine genes that had been shown to be expressed in cartilage.
An additional 3,248 random probes were placed on the array for quality control.
Probes are placed on the array in a rectangular grid of 'features', each containing many copies of the same oligo.
A cover slip was placed on the array and the cDNA mix was introduced between the cover slip and the array surface.
Standard NimbleGen probes were also placed on the array, including alignment probes, chip identification probes, and random cDNA probes designed to match the content of our user designed probes.
