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The major technical limitation arises from the need of coaxial placement of optical illumination and ultrasonic detection paths.
The hybrid nature of optoacoustic imaging might impose limitations on concurrent placement of optical and ultrasonic detection components, especially in high resolution microscopic applications that require dense arrangements and miniaturization of components.
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Figure S3 shows the location of optical fiber placement in each experiment.
To date, most of the group-level analysis in optical imaging is done either based on selection and averaging of regions-of-interest or based on direct averaging in the native measurement space (e.g. optical source-detector pairings) and requires careful reproducible placement of the optical probe.
The placement of the optical fiber or light guide in relation to the LED is important to consider.
In the first example, we will present the case of estimating functional activities of a single subject with data simulating the imperfect placement of the optical probe over multiple sessions.
In this first example, we simulated the repeated placement of an optical probe on the Colin27 atlas brain in order to examine the reconstruction errors introduced by a nearest-neighbor probe.
Using the proposed surface based method, optical image reconstruction in the space of this inter-subject registered surface provides a means to perform group-level averaging while accounting for both differences in the anatomy of the brain and placement of the optical sensors between subjects.
Thus, region-of-interest based group analysis can be used to indicate that two groups are different, but cannot be used to elicit the exact nature of this difference— magnitude of change, location of signal, or anatomical differences between subjects (e.g. systematic errors in the placement of the optical probe).
In order to achieve this we implemented an efficient approach to merge single map results from SOMA and an overview of this approach is provided below: Input = A set of contig placements on optical maps. 1. Select a contig placed on all the maps as an anchor.
While ChR2-EYFP expression extended beyond these two nuclei, targeted stimulation of the intralaminar nuclei was achieved by (a) stereotactic placement of the implanted optical fiber, as confirmed with high-resolution T2-weighted structural MR images, and (b) spatially restricted illumination.
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