The proposed planner automatically determines the pose of an object that is stably placed near a user-assigned point on the environment surface.
To determine the significance of cation binding to DNA stability, we designed a modification that stably placed a cation at a position in the major groove similar to that of diffusible cations observed in the crystal structures.
Reaction volumes were 20 μl and also included 1× Power SYBR® Green PCR Master Mix (Applied Biosystems) and either of the primer pairs listed in Additional file 1. Importantly, primer pairs were selected to target stably integrated regions not thought capable to excise from their respective places on the genomes.
Two explants were used to generate suspension cultures: stably transformed explants were placed on MS medium supplemented with 0.5 mg/l 2,4D (2,4-dichlorophenoxyacetic acid) and 40 mg/l kanamycin for the induction of callus, which was transferred into liquid MS medium supplemented with 1 mg/l 2,4D and 50 mg/l kanamycin.
Batches of the stably transfected cell lines were frozen in liquid nitrogen, and individual aliquots of the stably transfected cells were not placed in culture for more than 1 month.
To identify in planta PSKR1 phosphorylation sites, a C-terminally GFP-tagged full-length PSKR1 sequence was generated, placed under control of the CaMV 35S promoter and stably expressed in wild-type plants.
To assess whether BRCA1-IRIS plays a role in these phenotypes as well, we plated MDA-MB-468 cells stably expressing shcontrol or shIRIS inside inserts placed in the middle of wells of 6-well plates.
32 Subjects were placed in front of a block 7.5 cm high, positioned stably against a wall.
Approximately 1 × 105 glioma cells stably expressing green fluorescence protein (GFP) were gently placed (0.5 μl transfer volume) on the brain slice in 4 6 replicates per condition.
To distinguish between these two hypotheses ES cells were stably transfected with a construct where GFP expression was placed under the control of the promoter of the mesodermal marker brachyury [35].
