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Then the ants were placed on sections of pitcher wall, which were pulled out from underneath the ants' feet.
I was re-admitted and placed on sections.
Primary monoclonal antibody anti-human collagen type I (Sigma Aldrich, Poole, UK) was diluted 1 1000 in TBS/T then placed on sections and incubated overnight at 4 °C.
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Sixty microliters working-strength DNA ligase solution, 54 μL biotin-labeled oligonucleotide, and 6 μL T4 DNA ligase were placed on the sections, and the sections were incubated in a humidified chamber for 14 hours at 20°C.
Matrix solution without tiotropium was placed on tissue sections as control to check for matrix derived peaks interfering with the mass of the drug.
The primary antibody raised against a 200 kDa neurofilament polypeptide (mouse anti-pig, Amersham, UK) was diluted 1∶200 in immunodiluent and placed on the sections.
A solution of alkaline phosphatase labeled anti-digoxigenin antibody in 10% FCS (1 250) was placed on the sections for 60 min at 37°C.
Mouse monoclonal anti-BMP-7 antibody (12G3; 1 100 dilution; Stryker Biotech, Hopkinton, MA, USA) was placed on the sections overnight at 4°C.
A ready-to-use Spot-Light EGFR probe (Invitrogen) 15 µl was applied to the coverslips, which were placed on the sections, and the edges were sealed.
The total number of cells positively stained by each marker was determined by counting multiple grids randomly placed on tissue sections at medium power field magnification (40×).
Mouse monoclonal antibody against human type II collagen (1 1,000 dilution; Daiichi Fine Chemical, Toyama, Japan) was placed on each section for one hour at room temperature.
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