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Alternatively, wild-type tissues were also placed on medium containing 50 μM of 17 3-al, 50 μM 2-HOT or a combination of both.
Roots were cut from these sterile plants and placed on medium containing 1/2 MS salts, Gamborg's B-5 vitamins (Gamborg et al. 1968), 1 % sucrose, 1.0 ppm 2,4- d, 0.1 ppm BAP and 0.2 % gellan gum, pH 5.5.
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For various incubation times, these were the relative times that the stigmas were placed on the medium, the ovules were placed on the medium, the stigmas were pollinated, and imaging was started.
Seeds were placed on solid medium consisting of ½ MS (Murashige and Skoog basal medium, Sigma) with 1 % sucrose, 0.1 % vitamins (Murashige and Skoog, Sigma).
Cells are placed on the medium when they are ready for transmission and then transmitted independently of each other.
For isolated embryo culturing, the embryos were placed on MS medium and incubated at 28°C in dark or light.
Seeds were then rinsed several times in sterile distilled water before being placed on agar medium or on filter paper moistened with sterile distilled water in Petri dishes.
Exposure to light was found to increase the proportion of TEs differentiating in P. radiata callus cultures placed on induction medium.
400 µl of C601+H99 were plated onto 0.45 µm Biodyne® A membranes (PALL Life Sciences, Cat. no. 601012) placed on induction medium agar plates containing 200 µM acetosyringone.
When diploid S. cerevisiae is placed on sporulation medium it undergoes meiosis and packages four spores into an ascus (a round protective sac).
Hypocotyls were placed on MSCP1 medium.
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