A group of senior geneticists have called for international safeguards to apply to researchers who want to develop gene drives, with strict security measures placed on laboratories to prevent the accidental escape of "supercharged" GM organisms that are able to spread rapidly in the wild.
For water loss assay, leaves of control and OsPYLs transgenic plants grown under normal conditions were detached from 3-week-old seedlings and weighed immediately on a piece of weighing paper, and then placed on a laboratory table and weighed at indicated time intervals.
Petri dishes with inoculated leaf segments were routinely placed on a laboratory bench and maintained at 21°C to 26°C with a 16 h photoperiod.
The control computer, hardware interface, power supply units, and nectar reservoirs were all placed on a laboratory cart outside the back of the cage and connected to the feeders via signal cables and main nectar tubes.
To extract absorbed solvent molecules, 2 mL carbon disulfide was applied to the monitors, and they were placed on the laboratory bench for 30 min and given an occasional shake.
For each experiment, one practitioner was escorted into a treatment room and observed to ensure that a minimum distance of 10 centimeters was maintained from the treatment box that had been placed on the laboratory bench.
For water loss measurement, rosette leaves were detached from 4-week-old plants and weighted immediately on a piece of weighting paper, which was then placed on a laboratory bench (at a relative humidity of 40%to50%0% and a temperature of 22°C to 23°C).
However, increased burden has been placed on individual laboratories for assuring good manufacturing practice (GMP) through heavy documentation of each step en route to the clinical examination.
Currently, the burden of defining these cutoffs is placed on individual laboratories, and as a result, clinically relevant values still vary from center to center.
