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Cell lysates were placed on ice for 10 min.
All samples were immediately placed on ice for transfer.
Before adding the radioligands to the wells, the plates were placed on ice for 30 min.
Media was then transferred to 15 ml centrifuge tubes and placed on ice.
Blood samples (3 4 mL) were collected in heparinized tubes and placed on ice.
The urine was collected into a sterile polypropylene container, and immediately placed on ice.
In all experiments, the cells were placed on ice before flow cytometric analysis.
The reaction was incubated at 37 °C for 15 min and then was placed on ice.
The lysed cells were placed on ice.
Cells in 6-well plates were placed on ice.
Then the samples were placed on ice for five minutes.
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