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Human-derived osteoclasts were placed on a coating with refined topography and compared to dentine, a polished coating and polished sintered HAp.
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This technique involves drawing with a pencil on a sheet of paper placed on a copperplate coated with an extremely soft, sticky ground.
Bacteria were incubated for 2 h at 37°C in incubation buffer with the chemokines placed on a carbon coated copper grid and negatively stained as described by Engel and Furthmayr [21].
The samples were placed on a carbon coated copper grid to leave a thin film on the grid and negatively stained with 1% phosphotungstic acid.
First, the cells are placed on a plastic dish coated with a gelatinous mix of collagen and other proteins.
For TEM analysis, a drop of the nanoparticle suspension was placed on a formvar film coated on a copper grid.
Drop of colloidal solution was placed on a copper grid coated with a thin amorphous carbon film on a filter paper.
Sample was dried by rinsing with anhydrous acetone, placed on a sample holder, coated with silver before being scanned under vacuum.
After 5 minutes several seminiferous tubules fragments were placed on a clean slide coated with 1 mg/ml poly-L-lysine (Sigma, St .Louis, Missouri, United States) with a small drop of fixative, and the tubules were gently minced with tweezers.
Briefly, a 20 μL of cell suspension was placed on a cover glass coated with 0.1% poly-L-lysine.
The sample was placed on a copper grid coated with formvar-carbon (Agar Scientific Ltd., Essex, UK), dried overnight in an oven at 27°C, and observed on transmission electron microscopy TecNai G2 Spirit TEM (FEI).
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