Sentence examples for placed in frame from inspiring English sources

As before, the GFP sequence was placed in frame with the authentic viral initiation codon.

mCherry was placed in frame after the endogenous cpoB gene in the chromosome of E. coli MC4100 (LMC500); gfpmut2 was placed in frame before the tolA gene (see 'Bacterial strains and plasmids', above).

The chimeric construct including the CaMV 35S promoter that controls the nucleoplasmin coding region placed in frame with the coding region of apoaequorin (15) was obtained as an EcoR1 fragment from the pCon3 vector kindly provided by A. van der Luit (Amsterdam, The Netherlands).

A synthetic microsatellite (16.5 copies of the GT dinucleotide) was placed in frame with the URA3 gene and integrated at ten locations across the yeast genome and loss-of-function ura3 mutants were selected by growth on 5-fluoro-orotic acid (5FOA) (Hawk et al. 2005).

The chimeric construct including the 35S promoter controlling the nucleoplasmin coding region which was placed in frame with the coding region of apoaequorin was originally prepared by Badminton et al. (15) and was subcloned in the pCon3 vector kindly provided by A. van der Luit (Amsterdam, The Netherlands).

In both cases, the POI is normally placed in-frame between the N-terminal signal sequence and the mature form of the viral protein.

With the exception of pVI display, which has been evaluated for use with cDNA libraries [5], it is common to the majority of phage display protocols that the heterologous peptide is placed in-frame between an N-terminal signal sequence and the mature form of the viral capsid protein.

DNA fragments containing Ulp1 under the control of its endogenous promoter were amplified from yeast genomic DNA and placed in-frame with a carboxy-terminal GFP-tag in the CEN/LEU2 plasmid pAA3 [ 56].

To achieve this goal, we developed a system in which single cells express a gene – e.g. Gal4 or oncogenic H-RAS – only when the ORF is placed in-frame after an in vivo frameshift mutation.

For the fusion constructs, ELP and HFBI are placed in-frame upstream of the c-Myc tag in the pCaMGate vectors, with a (GSSS 3 linker separating AnPGI from either ELP or HFBI.

This was then recombined with either Ubq (R.B., unpublished data) or UASp (T. Murphy, personal communication) plasmids so that each coding sequence was placed, in-frame, with GFP at either their N or C terminus (full cloning details are available upon request).