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However, the declines may have been larger if greater effort was placed in activities that targeted reductions in injury.
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Animals were placed in activity monitors or in the two- compartment black and white box.
Females of each genotype were placed in activity monitor tubes and acclimated for 1 day.
Freshly collected animals were individually placed in activity recording tubes in DD containing 2 ml of seawater and PF670462 at a final concentration of 25 μM.
Animals were placed in activity cages (Versamax, AccuScan Instruments) with water and food available and distances travelled were monitored for a period of 24 h.
Individual male mice (26 B−/− and 17 WT) were placed in activity chambers for 60 min. Locomotor activity was measured in a 41 × 41 × 30 cm Accuscan activity monitor equipped with 16 pairs of photodetector-LED beams along the x and y axes (Accuscan Electronics with VersaMax software, Columbus, OH).
In these studies, reference females and females corresponding to multiple yar genotypes were placed in activity monitor tubes, preconditioned for 3 days of 12-hr light and dark cycles, followed by 12 hr of mechanical sleep deprivation during the fourth night.
Mice were placed in voluntary activity wheels for 3 days before the start of injections, as well as 2 weeks following treatment, as an indicator of physical activity.
Mice were injected with a saline solution and individually placed in the activity box for 30 min during 3 days consecutively for habituation before all locomotor experiments.
Behavioral testing started when the animals were placed in the activity boxes for a 60-min habituation period.
When placed in an activity chamber for 30 min for the first time, 1-year-old control male and female mice exhibited similar levels of exploratory activity.
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