In order to get wild type cDNA sequence, we then used the high-fidelity thermostable DNA polymerase Phusion DNA polymerase (New England Biolabs) to amplify the target DNA sequence in place of error-prone Taq polymerase.
Pierce and Denison (2006) assessed place-of-residence errors on death certificates in only two Texas counties and found a 14% error rate in recording county of residence for deaths [ 38].
The Chinese articulation test was used to evaluate the place and manner of error production, and a questionnaire on dietary habits was used to evaluate deglutition 6 months to 10 years after reconstruction.Results: Patients with the free flap had more intelligible speech.
Asked how long he could afford to stay at his "margin-of-error" place in the polls, he said, "Give us a month or two and I think we'll be punching through".
For hearing impaired listeners, it has been shown that places of articulation errors are more prevalent followed by errors in the manner of articulation [ 7, 8].
Every time that this process takes place, the overall rate of error is reduced.
There is also a risk of AEs never being recorded in the first place, but this type of error would most likely affect less severe AEs.
Remember, though, that fewer people are surveyed in such small places, so the margin of error is much greater.
