In this paper, we present a case study in which we constructed a user interface for "pick-and-place configuration" of the Flexible Assembly Unit FAU.
In the third exposure, control animals explored the now familiar objects but in a rearranged object-place configuration.
Eight days later, the experiment was repeated with the now familiar object-place configuration (panels 3 in Fig. 4; results in Fig. 6).
Thus, MPEP prevented learning of the new object-place configuration, and MPEP-treated animals behaved, upon reexposure to this environment, as if they had never experienced it before.
Here, a similar effect was seen, but in MPEP-treated animals, no habituation was evident when animals explored the object-place configuration for a second time (Fig. 10).
The responses of the control animals replicated the effects seen in the previous experiments: first exposure facilitated LTD, and reexposure to the same object-place configuration did not.
Animals responded upon reexposure to the same object-place configuration as if they had never seen this configuration before, and under these circumstances, LTD was facilitated.
A subsequent (third) exposure to the same object-place configuration failed to facilitate LTD and revealed that the animals had now habituated to the environment.
Whereas reexposure to the object-place configuration (after 7 days) failed to facilitate LTD in control animals, those who had been treated previously with MPEP expressed LTD, suggesting that inhibition of learning contributed to the initial prevention of LTD.
Here, LTD was not facilitated when animals explored the "same" object-place configuration for a third time (Fig. 6; ANOVA compared to previous exposure to familiar hole board: F1,257 = 342.94, P < 0.0001).
