Major applications covered include assays, impurity testing, high-throughput screening, chiral separation, pKa determination, ion analysis, impurity profiling, orthogonal method, and characterization of proteins, peptides, and nucleotides.
pKa calculations and analysis of mutant mannanases further revealed that the location of Gln91 and Glu226 render BSP165 MAN active under high pH conditions, and mutations at the two sites might be involved in an evolutionarily critical change in the pH optimum for the activity of GH5-8 mannanases.
The following are among the genes upregulated in MB compared to CB and TG in all three species that are known to be involved in signal transduction: Shaggy, CAMKII known to be highly expressed in honey bee mushroom bodies by in situ hybridization analysis [50], Pka-R2 and Pka-c code for the regulatory and catalytic subunits of cAMP dependent protein kinase or PKA.
Tautomeric ambiguities for all data set entries were resolved using the pKa predictions and microstate analysis in ADMET Predictor 7.0.
Activation of the RIαB allele in vivo provides a novel system for the analysis of PKA function in physiology.
Two amino acid substitutions in the vicinity of the acid/base catalyst were proposed to be possibly responsible for the variation in pH optimum of these homologous enzymes in subfamily 8 of GH5, identified by sequence homology analysis and pKa calculations of the active site residues.
Sensitivity analysis supported PKA activation as the primary means of regulating the steroid response under stress.
The developed chip makes great advancement towards real use of chip in acid- base titration, EOF monitoring, pKa determination and protein content analysis.
The mass action kinetic model has been extended to allow the analysis of the pKa for HA activation and pKi for HA inactivation.
The EOF-free chip could be well used for: (i) acid-base ET; (ii) EOF monitoring; (iii) pKa determination; (iv) protein content analysis.
