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From the origin (at x, y, z = 0), we extract seven (7) three-dimensional subsample image windows of 50, 100, 200, 300, 400, 500 and 600 μm pixel lengths.
Pixel lengths of small intestine villi were measured on the H&E-stained cross-sections by using ImageJ software (http://imagej.nih.gov/ij/).
Villus lengths were calculated from the pixel lengths of multiple well-oriented representative villi for each small intestinal cross-section using a 10-μm scale.
Physical length measurements were taken for each chromosome using GIMP 2.6 and CHIAS IV imaging software and by converting pixel lengths into microns.
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A set of pixels with the same gray level in each row is called a stroke and each stroke only records the gray level and the pixel length.
d Graphical annotation design of six (6) REV image windows of 50, 100, 200, 300, 400, 500 and 600 μm pixel length.
Firstly, as can be seen in Figure2, large-scale variations over small pixel length scales in the projected weld geometry dominate the gray scale variation compared to the pore indications shown in Figure3.
The "window" was a circular region with a 51 pixel length (1.3 cm) radius, covering a total of 8171 pixels.
The distance between the vertebrae was determined by finding the pixel length of the vertebrae and the pixel length of the gap between their left neighbouring vertebrae.
From each experimental image, 10 lines of 100 pixel length were extracted.
Quantification carried out by calculating total pixel length reflects the lack of tubule formation.
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