Nuclear extraction was performed by adding 500 μl PIPES buffer (5 mM Pipes pH 8.0, 85 mM KCl, 0.5% Nonidet P-40, protease inhibitors), incubating for 10 min on ice and removing cytoplasmic components by centrifugation.
Of the Au NPs synthesized in this paper, NPs synthesized using the PIPES buffer have the highest zeta potential (−36.1 mV) and show the greatest stability due to the strong surface adsorption of the PIPES buffer onto the Au surface.
In particular, both theoretical and experimental observations agreed on the long-term stability of Au NPs formed using PIPES buffer.
The results revealed that Au NPs synthesized using the PIPES buffer have the highest zeta potential and highest interaction energy, producing the most stable NPs of the group.
A 10-mL fixative (4% paraformaldehdye, 2.5% gluteraldehyde in PIPES buffer, pH 7.2) was added to the flasks and incubated for 0.5 h at room temperature.
Glucose oxidase (GOD) was entrapped in a polypyrrole film during the electropolymerization of a pyrrole solution containing PIPES buffer (pKa = 6.8), LiClO4 and sodium dodecylsulphate.
Briefly, the enzyme reaction was performed using p-Nitrophenyl-alpha-d-glucopyranoside (PNP-glycoside) as a substrate in 0.1 M piperazine- N,N′-bis (2-ethanesulfonic acid) (PIPES) buffer, pH 6.8.
Four milliliters of 100 mM aqueous PIPES buffer and 1 mL of 20 mM aqueous HAuCl4 were placed in 36 mL DI water and vigorously stirred at 25 °C.
The optimized conditions for the sensor response were obtained in 0.25 mol dm−3 Pipes buffer (pH 7.0) containing 150 μmol dm−3 H2O2, with an applied potential of −50 mV versus saturated calomel electrode (SCE).
A simultaneous resistive transition of a LHC magnet sector, which may occasionally occur, will cause rapid helium outflow from magnet cryostats to a special relief system composed of long pipes, buffer volumes, and accessories.
The cells were then incubated for 10 minutes in macrofixative (0.1% Glutaraldehyde, 0.5% NP-40 in potassium Pipes buffer).
