Twelve derivatives of BAF11 were scanned to pinpoint the essential apolar and polar interactions for the pharmacorphore refinement.
To further pinpoint the essential Ser for the KDAC activity, we incubated WT YcgC with hydrolase inhibitor AEBSF and the following MS/MS analysis revealed that Ser200 was the only residue covalently labeled with AEBSF (See new Figure 3B).
Characterizing the many lineages known only from environmental rRNA sequences, establishing the full metabolic diversity of the group, and extensive genomic and evolutionary studies to determine whether it is holophyletic or paraphyletic are all essential for finally pinpointing the root of the tree of life.
The show notes included a map pinpointing the birth place of the models and their parents: 'Essential to my show concept is a global view on diversity linked to the travel dna of the brand,' Abloh explained.
(The delayed autopsy made pinpointing the time of death impossible).
Pinpointing the initial molecular trigger, however, is difficult.
To further narrow down to the interacting domains, we created smaller GST-tagged TRIM24 truncation mutants of the N-terminal region and second-round of GST pull-down pinpointed the B1B2 domain (aa100 260) as essential for TRIM28 interaction (Fig. 2e).
Finally, as suggested by Murray et al. ([2012]), while investigating test takers' attitude, it is essential to pinpoint the sources of the positivity and negativity in their reported attitude.
To elucidate the significance of this in vivo characterized catalog of human enhancers in organismal development, physiology and medicine it is essential to pinpoint the precise target gene for each of these elements.
Since microbial metabolism depends on the interaction of hundreds to thousands of metabolic reactions, systems-level modeling approaches that can incorporate existing experimental data are essential to pinpoint the factors that play a crucial role in microbial biofilm formation.
At this point, it's essential to pinpoint the single, strong idea you'll be discussing, your assertion that you believe you can defend throughout the paper and that makes it clear to a reader what they're about to learn about and be given a sound conclusion on.
