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The microarray was printed on poly-L-lysine slides (Thermo Scientific) in a 48 pin format using an OmniGrid-100 arrayer (GeneMachines).
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The arrays were manufactured by an OmniGrid arrayer (GeneMachine, San Carlos, CA, USA) using a 24-pin format.
The arrays were manufactured using an OmniGrid arrayer (GeneMachine, San Carlos, CA) using a 24-pin format.
Array production – All A. burtoni cDNA clones (including 1185 that failed the gel analysis above) and 120 control clones were spotted in duplicate arrays onto NaOH cleaned, poly-lysine (Sigma) coated slides using the 16-pin format on an OmniGrid-100 arrayer (GeneMachines).
That includes a way to pin documents, launch presentations, format text and insert docs and stickers.
Still, Pedowitz stressed the CW isn't aiming to be viewed as a genre network -- noting the addition of "Jane the Virgin," based on a Venezuelan telenovela about a hardworking religious girl who is accidentally artificially inseminated; as well as its continued effort to pin down the comedy format.
Comparing cell density estimates immediately after inoculation with estimates in stationary phase for robustly growing, 1536-format, pinned cultures, we estimate that the maximum dilution achievable at each passage with this method is 16-fold.
We dissolved chemicals with dimethyl sulfoxide (DMSO) into eight stock concentrations transferred into 1,536-well 1,536-wellat via a plateool station (Kalypsys Inc).
The barFLEX array was pinned in a 1536 strain format onto glucose- and galactose-containing plates and colony size was assessed using an automated colony scoring method (Tong et al. 2004).
Phage lysate of each of the 14 candidate antigens as well as β-gal and human IgG control antigen was diluted in SM buffer at 2 × 10 pfu/μl and dotted in triplicates on XL1 bacterial lawn in 96-well format using 96-pin replicator (Molecular Devices, Sunnyvale, CA, USA), and incubated with IPTG-treated nitrocellulose membranes for 12 h at 37 °C.
As Phillips explained it, Waze Local's "core ad format" is the branded pin, which will appear on users' screens as they drive near a store's location.
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