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Plus, the Jumping Sumo comes equipped with a wide angle, built-in camera that captures video and streams live views to the piloting screen.
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The assay had high sensitivity and excellent reproducibility, which were validated in a pilot screen (Fig. S1A and S1B).
The TR FRET assay is suitable for high-throughput screening (HTS), as shown by performing a pilot screen of the National Institutes of Health NIH Clinical Collection Libraryry in a 384-well plate format.
Through a pilot screen of 2802 compounds from the National Cancer Institute (NCI) chemical libraries, we identified aclacinomycin (Acm) as a compound that preferentially affects ER function.
A pilot screen of a pharmacologically characterized compound library identified two thiol-modifying compounds as authentic NDM-1 inhibitors: p-chloromecuribenzoate (p-CMB) and nitroprusside.
After validation of the assay by using 5-HT3 andnists antagonistsists of reference, a pilot screen enabled us to identify azelastine, a well-known histamine H1 antagonist, as a potent 5-HT3 antagonist.
Finally, we performed a pilot screen using 80 kinase inhibitors.
In our pilot screen we found that reduction of apkc or crb substantially enhances the baz mutant cuticle phenotype.
We propose that this pilot screen, using in parallel the three cell lines discussed above, provides us with proof-of-concept for a larger screening campaign to follow.
In a pilot screen we demonstrate that anterior segment and fundus alterations can be found in living adult zebrafish carrying novel (induced) genetic mutations.
In order to find lens and retinal alterations, a pilot screen of 299 families of the F3 generation of ENU-treated adult zebrafish was carried out.
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