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More accurate values of α and γ can be obtained from pilot screens, which are often carried out anyway, where these parameters can be measured directly by keeping track of the amount of work done (time spent) to pick a fixed number of F1 animals and pick and score a fixed number of F2 animals.
We then performed automated pilot screens of 1280 compounds (LOPAC library) using the Fas/FADD/Caspase-8 and NLRP1ΔLRR/Asc/Caspase-1 achievinghieving an average hit rate of ∼0.5 and ∼0.5 per 384 well plate, respectively, which is quite acceptable particularly given that the library is enriched in bioactive molecules.
Systematic, qualitative phenotyping by dedicated, expert anatomists has also proved feasible in pilot screens.
In pilot screens, adult fish were outcrossed to brass for ease of husbandry.
These pilot screens generated 234 lines of which 45 insertions result in protein traps in 33 unique genes.
Before the actual library screen, several small-scale pilot screens were carried out to optimally adjust the screening conditions.
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Spohr said that despite the disaster, Lufthansa had full confidence in its training and pilot screening procedures, which would nevertheless be reviewed.
In particular, the centers are seeking to develop a comprehensive surveillance system for the disease, organizing pilot screening projects for people at high risk in California, Florida, Minnesota and New York.
Through a pilot screening program, Giana was found to have an inherited metabolic disorder called 3-MCC (3-methylcrotonyl-CoA carboxylase deficiency).
The assay had high sensitivity and excellent reproducibility, which were validated in a pilot screen (Fig. S1A and S1B).
The TR FRET assay is suitable for high-throughput screening (HTS), as shown by performing a pilot screen of the National Institutes of Health NIH Clinical Collection Libraryry in a 384-well plate format.
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