Exact(5)
Designed the EMS pilot dose experiments, selected the doses and checked the final manuscript.
Pilot dose responses were performed using the NR assay to assess an approximate lethal dose 100% (LD100) of SSP and H2O2 in both cell lines.
In a pilot dose finding study performed for each tumour type the melphalan dose inflicting a partial tumour response was chosen for this study.
Due to differing transduction efficiencies among cell lines, each cell type was infected at an optimized MOI, based on pilot dose response studies conducted with the Ad/ GFPTET vector.
Three of the 19 P450 cDNAs were found to be significantly induced in the permethrin treated ALHF house flies after 24 hours treatment (Fig. 1) at the pilot dose of LD50 that caused ~50% mortality of each house fly strain.
Similar(55)
To determine optimal experimental conditions, a pilot dose-dependent titration experiment was performed exposing Pods to Cur concentrations ranging from 1-100 μM.
As this is a pilot dose-finding study, without precedence on which to base accurate power calculations, a power calculation has not been performed.
The concentrations of these agents were established in pilot dose-response experiments to perturb the relevant cytoskeletal system without preventing cell attachment or reducing cell viability over an 18 h period.
We determined that 33 μg/L is a biologically relevant dose through pilot dose-response analysis where we treated individuals for two weeks as described above but at four different concentrations (Additional file 1: Figure S1).
Here, pilot dose-finding and clinical outcome data are needed to inform the design of rigorous intervention trials should pilot studies show potential benefits of vitamin D supplementation in ICU patients.
In the pilot low dose determination study, 2.5 mg of oseltamivir was determined to be the low dose.
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