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Thus, a larger clinical study appears warranted given the partial positive outcome of this pilot assay.
Therefore, the results of this pilot assay point to accumulation and functional relevance of the infectious-virus-like sequences of the human genome.
In a pilot assay of Hoechst 33342, a rapid assay based on fluorescent detection of compacted chromatin in apoptotic cells, MGC-803 cells were incubated with 50 μg/mL of EAC-B by 0, 2, 8 h.
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Moreover, our pilot assays in mice have suggested that this metabolite accounts for approximately 50%% of radioactivity in the brain at 30 min after intravenous injection of [11C]TASP0410457 (K. Kawamura, personal communication, 2015).
CLEAs preparation: The pilot assays yielded optimal enzyme precipitation when propanol was used at precipitant/enzyme solution ratio 4∶1.
The pilot assays yielded optimal active CLEAs when propanol was used at a ratio of 4/1 with a 2 h cross-linking period at 0.5% glutaraldehyde.
To test the validity of the parameters found in the small-scale pilot assays of CLEAs preparations, we scaled up the procedure to a 100-fold.
After isobaric tagging, each fraction was pooled 1 1 and tested in a pilot analysis; the pilot assays were used to devise appropriate multiplex pooling mixtures.
Each limited the transport of inert 3 kDa and 70 kDa dextran molecules when used in transwell permeability pilot assays for at least 8 hours.
Pilot assays showed that NSV particles exposed to heat or UV light did not trigger microglial cytokine or chemokine production (data not shown).
The IPK-OPA has been mainly built on a selection of markers from two pilot assays (pOPA1, pOPA2) that are polymorphic between the two barley cultivars 'Barke' and, 'Morex'.
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