Sentence examples for pig secondary from inspiring English sources
Membranes were subsequently incubated, without stripping, with anti-OBP48 antibody at a 1∶1,000 dilution and a 1∶1,000 anti-guinea pig secondary antibody.
Alexa Fluor 488-conjugated goat anti-rabbit and Alexa Fluor 555-anti-guinea pig secondary antibodies were used at 1∶400 dilution.
The primary antibodies were detected with Cy3-conjugated anti-rabbit Alexalexa 488-conjugated anti-guinea pig secondary antibodies (1/2000, Molecular Probes).
Immunoblotting was initially performed with anti-OBP1 antibody at a 1∶800 dilution and a 1∶1,000 anti-guinea pig secondary antibody (Jackson ImmunoResearch Laboratories, Inc ., using the Amersham ECL Western Blotting detection reagents (GE Healthcare) or Pierce SuperSignal West Pico chemiluminescent substrate (ThermoScientific).
FITC-conjugated goat anti-mouse and TRITC-conjugated goat anti-guinea pig secondary antibodies were used respectively to visualize the caveolin-2 and EBs within the cells.
Endogenous Trim17 was detected using the affinity purified anti-peptide antibody, and microinjected neurons were visualized with fluorescent anti-guinea pig secondary antibody.
Sections then received three washes in PBS before incubation in biotinylated donkey anti-Guinea pig secondaries (1 250 in PBS, Jackson Immunoresearch, USA) for 4 h at room temperature.
Freeze-fractured sections were incubated in Guinea pig anti-VGLUT1 (1:10,000 in PBS, Millipore, UK) overnight at 4 °C and then in biotinylated donkey anti-Guinea pig secondaries (1 250 in PBS, Jackson ImmunoResearch, USA) for 4 h at room temperature, with three washes in PBS between each incubation.
After thorough PBST washing, sections were incubated with Alexa Fluor 568 donkey anti-guinea-pig secondary antibody diluted in PBST + 2% normal serum (1 2,000, one hour, room temperature, Molecular Probes, Eugene, OR, USA).
Sections were then incubated in Texas Red-conjugated goat anti-guinea pig Ig secondary antibody together with FITC-conjugated goat anti-rabbit secondary antibody (Vector).
Astrocytes were then washed three times in PBS and incubated for 90 min at room temperature with a combination of a donkey anti-rabbit CY2 and a donkey anti-guinea-pig CY3 secondary antibody (1∶200 dilution for each, Jackson Immunoresearch, West Grove, PA).
