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Although neonatal human sera were not cytotoxic to target cells, adult sera were cytotoxic to both pig aortic endothelial cells and pig lymphocytes.
A whole cell enzyme-linked immunosorbent assay and a complement-mediated cytotoxicity assay were performed to determine the binding and cytotoxicity of these xenoantibodies to pig aortic endothelial cells and pig lymphocytes.
Pig lymphocytes were cultured at 39 °C in 7.5% CO2 humidified air atmosphere.
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We investigated the possibility to induce tolerance against pig peripheral lymphocytes (pPBL) in galactosyltransferase knockout mice (gal -/-), which produce antibodies against Galalpha1-3Gal.
NK-lysin is one kind of host defense peptide (HDP) produced by pig cytolytic lymphocytes.
Open image in new window Fig. 3 a Mean ± SE of stimulation index (SI) of guinea pigs (peripheral blood lymphocytes) subcutaneously implanted with native (I), acellular (II), BDDGE (III) and EDC (IV) cross-linked aortic grafts at day 0, b at day 15, c at day 60.
To establish the in vivo effectiveness of CsA (Sandimmun Neoral® Novartis, Basel, Switzerland), we prepared the pigs' peripheral blood lymphocytes (PBLs) and cultured them in vitro with or without proliferative agents.
However, low level binding of immunoglobulin G xenoantibodies was detected to pig endothelial cells and lymphocytes.
Our findings indicate that neonatal human sera lack natural antipig immunoglobulin M xenoantibodies, and therefore, neonatal human serum is not cytotoxic to pig endothelial cells or lymphocytes.
As shown in Table 5, the percentages of CD3+ CD4+ CD8− (P < 0.01) and CD3+ CD4− CD8+ (P < 0.001) T lymphocytes in pigs from G2 were significantly lower than those from G1, G3, and G4, and the highest was found in pigs from G1.
The other direction of leukocyte migration is also possible: It has been documented in pigs and rodents that lymphocytes can return to the lung interstitium and then reach the regional lymph nodes [ 4].
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