Sentence examples for pig embryonic from inspiring English sources
Genetic modification of the pig has been hampered by inefficiency of homologous recombination and unavailability of pig embryonic stem cells.
The mESC-like piPSCs used in this study were generated from the pig embryonic fibroblasts and provided by professor Jianyong Han39.
Since there are no pig embryonic stem cells, pig genetic engineering is done in fetal fibroblasts that remain totipotent for only 3 to 5 wk.
In this study, we investigated the effect of 3D collagen scaffolds on the reprogramming of mouse embryonic fibroblasts (MEFs) and pig embryonic fibroblasts (PEFs).
These findings are in agreement with recent reports that mouse ESCs-like state piPSCs could contribute to pig embryonic development (Fujishiro et al., 2013).
The donor cells used for induction were pig embryonic fibroblasts (PEFs) that were obtained from 33.5 dpc embryos of Duroc strain.
Fatty acid metabolism appears to be unique to pig early embryonic development, and its function is unclear.
This might have resulted in the formation of more muscle fibers in Lde pig during embryonic development, which may explain the main phenotype difference in prenatal muscle development between the LT and Lde breeds [ 11].
The in vitro developmental efficiencies of embryos injected with Cas9 mRNA/sgRNA (∼79%) and embryos injected with water (∼77%) were both very high and comparable with each other, suggesting that the microinjection manipulation and the Cas9 mRNA/sgRNA had little effect on pig early embryonic development (Supplementary information, Table S1).
Gene-regulation networks of mouse PEDs have been extensively studied and reported [ 15– 17], but scarce information regarding molecular mechanism of pig early embryonic development as well as other large domestic animals has limited our knowledge of developmental biology and aspects of engineering their stem cells.
They identify 337 genes that are differentially expressed by affected and healthy pigs during embryonic development, thereby shedding light on the transcriptional network that is involved in HOXA1 -mediated development of the external ears.
