Sentence examples for pierce 1 from inspiring English sources
Total glutathione was measured by adding reducing agent TCEP (Pierce; 1 mM final concentration) to 3 separate aliquots of each suspension to convert GSSG to GSH prior to GSH measurement; the amount of TCEP added did not significantly alter the total volume.
Three µg of nuclear extract were incubated for 30 minutes at 37°C with 2.6 µl 5× Binding buffer (LightShift Chemiluminescent EMSA Kit, 20148X, Pierce), 1 µl poly-deoxyinosic-deoxycytidylic, and 1 µl of biotin-labeled wild type-attB or mutant attB.
Protein was extracted from L6 myotubes with RIPA (radioimmunoprecipitation assay) buffer, containing 50 mM Tris/HCl (pH 7.4), 150 mM NaCl, 1% NP-40, 0.25% Na-deoxycholate, 1 mM EDTA, 1% protease inhibitors cocktail (Pierce), 1 mM PMSF (Pierce), 1 mM Na3VO4 (Sigma) and 50 mM NaF (Sigma).
The liganded beads were blocked with excess biotin and washed with blocking buffer (SeaBlock (Pierce), 1% BSA, 0.05% Tween-20 and 1 mM DTT) to remove unbound ligand and to reduce nonspecific binding.
Pierce 1 beet with a table knife to check for tenderness.
Primary antibodies were detected with HRP-conjugated anti-rabbit (32260, 1 10.000, Grand Island, NY, United States), anti-mouse (32230, Pierce, 1 10.000), anti-chicken (31401, Pierce, 1 5000) or HRP-conjugated streptavidin (18-152, Millipore, 1 5000).
Williams *3-0 Pierce Wild forehand from Venus puts Pierce 0-30 up, but she comes back with a wonderfully timed one to leave Pierce standing.
Mr. Pierce, 43, learned about the car in 1994.
Pierce (5 of 12) was a nonfactor, again.
Allen (22 points) Garnett (18) and Pierce (14) contributed heavily.
Antoine Walker led the Celtics with 21 points; Tony Delk had 19 and Pierce 16.
