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The pieces were washed of the mud and vegetation that had clung to them through two centuries underground.
Once counted and categorized, the pieces were washed with distilled water, transferred to aluminium dishes, dried overnight at 60 °C, and weighed using an OHAUS Explorer EX324M (0.0001 g readability) for objects <5 cm, and a OHAUS Explorer EX12001M (0.1 g readability) for objects >5 cm.
The sample pieces were washed with distilled water for several times and dried it in an oven at 110 °C overnight.
After being washed in detergent water, the titanium pieces were washed in an ultrasonic cleaner for 1 h with deionized water and alcohol, respectively.
The gel pieces were washed twice with water.
Briefly, gel pieces were washed twice with 100 mM NH4HCO3/50% acetonitrile and once with acetonitrile.
After reduction, the gel pieces were washed 3 times with 50 mM NH4HCO3.
Finally, the gel pieces were washed with 100% ACN for 5 min, and then dried.
Gel pieces were washed in 50 mM ammonium bicarbonic acid containing 50% acetonitrile for 10 min, twice.
Gel pieces were washed 50 mM ammoniumbicarbonate/50% (v/v) methanol and with 75% (v/v) ACN.
Sliced gel pieces were washed with 100 mM NH4HCO3 and acetonitrile (1∶1, v/v) (buffer A).
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