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The gel pieces were subsequently washed in 40 µl of 30% ACN and 5% formic acid followed by a second wash containing 60% ACN and 0.1% formic acid.
The reaction was stopped by addition of 0.001% horse serum, and cortex pieces were subsequently mechanically dissociated.
The fine-cut scale pieces were subsequently weighed and wrapped into 6 × 4 mm tin capsules for elemental analysis (Elemental Microanalysis Ltd ,Okehampton, UK).
The tissue pieces were subsequently incubated at 37 °C in humid air with 5% CO2, and the medium was replaced every 3 days after the initial plating.
The gel pieces were subsequently dehydrated by washing with 200 μL of 100% acetonitrile and completely dried in a Speedvac concentrator.
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(The piece was subsequently given its concert debut January 23 by the Pittsburgh Symphony Orchestra).
Her piece was subsequently published in newspapers across the country.
The piece was subsequently sold to other individuals.
The piece was subsequently staged in San Francisco, New York and Milan during the next year, but failed to make a positive impression, and did not enter the regular operatic repertory.
Sepals and fruits from wild type and transgenic plants were cut into little pieces that were subsequently fixed in 3 % glutaraldehyde in 0.1 M cacodylate buffer (pH 6.9) for 24 h and post-fixed for 2 h in 1 % osmium tetroxide in 0.1 M cacodylate buffer, dehydrated in a graded ethanol series and then embedded in araldite resin.
On Friday the Metropolitan Police said that it handed its first file of evidence to the CPS on 28 March, but three pieces of information were subsequently added.
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