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The pieces were soaked in a solution of 85% phosphoric acid (H3PO4) at 1 1.75 (w/w%) impregnation ratio for 24 h at room temperature.
Briefly, sponge pieces were soaked in PBS, pH7.2.
Gel pieces were soaked in electroblot buffer for 5-10 minutes.
The aligned electrospun pieces were soaked in 75% ethanol to remove residual solvent and sterilized for 3 days.
After removing bark and pith, wood pieces were soaked in water for at least 48 h prior to volume measurement.
Gel pieces were soaked for 60 min in a solution of 2 μM modified trypsin (Promega, Mannheim, Germany) in 50 mM ammonium bicarbonate buffer pH 8 at 4°C.
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Another piece was soaked in alcohol for a tincture, the latter of which she still uses "like Rescue Remedy".
The resultant pieces (1.3 kg) were soaked in deionized water (3000 mL) and refluxed for 4 h.
In other unpublished experiments pieces of film were soaked in solutions containing 1 mM ADP, 5 mM KH2PO4, together with 100 mM NaCl, 100 mM MgCl2, 100 mM KCl or no added electrolyte.
To attract male E. frontalis, small pieces of paper were soaked with 1,8-cineole or methyl salicylate (source: Merck & Co. Inc., NJ, USA), fundamental compounds in orchid floral fragrances [23], [50], [51], and attached to tree trunks at ca. 1.2 metres height.
For the final structure, small laminated pieces of Douglas fir were soaked and then glued together to make curved ribs that snapped together, with fibreglass pods between, on the site.
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