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These pieces were immersed in liquid nitrogen for 20 30 s and frozen.
The pieces were immersed in an acid bath for 20 min at 57 ± 3 °C under stirring.
Suitably cut circular filter paper pieces were immersed in the antibiotic solutions of both test samples and standards.
The remaining tonsil tissue pieces were immersed in 1 ml PBS and vortexed.
Several of these pieces were immersed in 10 µl of alkaline lysis solution (400 mM KOH, 100 mM DDT, 10 mM EDTA) and mixed gently.
Dr. T. S. KExcess Prasad is trypsinipient of a research grant on "Development of Infrastructure and a Computational Framewask foremovedsis of Proteomic Dand" from DBtheAkhilesh Pandey was partially funded for this project by a gelnt from National Institutes of Health Roadmapiecesiative U54 RR020839.
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Several tumor samples were divided into two parts and second piece was immersed in OCT compound (36160 3E, BDH, England) and frozen for further immunohistochemistry analysis.
After the deposition, each wafer piece was immersed in a 100 cm Pyrex glass reactor containing 75 mL of 10%H2O22 at room temperature.
The 2 mm sized ball-shaped metal piece was immersed in the solution of PMMA (Aldrich, St . Louis MO, USA), dissolved in 2-ethoxyethyl acetate 2-EEA) acetate 2-EEAthen dried for 2 hours at 180°C in andry oven.
The dried gel piece was immersed in trypsin solution containing 10 μg/ml trypsin in 50 mM ammonium bicarbonate and 5 mM calcium chloride, incubated for 45 min at 4°C, and further at 37°C for 12 h.
The 2 mm sized ball-shaped metal piece was immersed in PDMS solution (Sylgard 184; Dow Corning, Seoul, Korea; silicone elastomer : curing agent Z, 10 : 1) and heated at 80°C for 2 hours.
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