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Shortly, after dehydration and resin (Durcupan ACM, Fluka) infiltration steps, dissected tissue pieces were embedded in hand-made silicone holders filled with 100% resin and clamped between two objective glasses.
Medical personnel said many people suffered from shrapnel-type injuries in their legs, though they were uncertain whether the metal pieces were embedded in the bombs or were blast debris.
The two agar pieces were embedded in paraffin under standard conditions for surgical biopsies.
Briefly, small tumor pieces were embedded in 5% low-gelling-temperature agarose (type VII-1; Sigma-Aldrich).
Subsequently, the tissue pieces were embedded in a mixture of epoxy resins and were allowed to polymerize in a thermostat.
The pieces were embedded in Durcupan (Fluka, Buchs, Switzerland).
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The lower piece was embedded in paraffin and sectioned to obtain longitudinal sections of the specimen.
Each piece was embedded in a paraffin block, cut in 4μm in thickness, and stained with hematoxylin and eosin (HE).
Formalin-fixed uterine pieces were embedded on end in paraffin, and cross sections were cut in 4-μm slices, mounted on Superfrost charged slides (Fisher, Pittsburgh, PA), deparaffinized, and hydrated.
Liver, colon and caecum were carefully dissected, weighted and immersed in liquid nitrogen before storage at −80 °C; pieces of liver were embedded in OCT compound and frozen in nitrogen-cooled isopentane for histology.
Small pieces of amber were embedded in epoxy resin and after polymerization each block was cut and finely polished [ 26].
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