Propagation - Both commercially and in the home, propagation can be achieved by using short pieces of stem, one to three segments long, twisted off rather than cut.
Remove any large, woody pieces of stem and discard.
Sift out the big pieces of stem, etc... so they don't clog up your applicator.
To test that idea, they pumped water into pieces of stems from laurel (Laurus nobilis), steadily increasing the amount of potassium chloride (KCl).
Small pieces of stems were fixed with 4%% (w/v) paraformaldehyde/0.1%% (v/v) Triton X-100 in phosphate buffered saline (135 mM, NaCl, 3 mM KCl, 1.5 mM KH2PO4, and 8 mM Na2HPO4) and embedded in polyethylene glycol 1500 (Merck, Darmstadt, Germany).
If they are still attached to the plant, you can cut the vine flush with the gourd or leave a piece of stem intact--leaving the gourd is purely ornamental when you dry the gourd on the vine.
Briefly, small pieces of dried stem (100 g) were boiled twice with 500 ml of distilled water for 10 min each.
Three weeks after eggs hatched, host larvae from the three species were taken from rearing vials and placed on fresh pieces of maize stem for 24 h, to ensure acceptance by the parasitoid (Overholt et al. 1994).
Seventy-two hours after inoculation, total RNA was extracted from 18 infected plants by cutting a 1 cm piece of pea stem containing the advancing lesion front toward the base of the plant using the TRIzol Plus RNA Purification Kit (Invitrogen, Carlsbad, California, USA).
The lilies, when touched by the urchin, lie down and use their feeding arms in the crown ("flower") at the top to crawl away, dropping off a piece of the stem to distract the urchin so that they can escape.
Normally a piece of the stem will grow a head end, or hydranth, at its free, or distal, end; if that is tied off, however, it regenerates a hydranth at the end that was originally proximal.
