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For some gilding operations the gilder uses a cushion to hold his pieces of leaf.
Larger soil invertebrate animals perform the function of biting off (shredding) pieces of leaf litter in forest soils and passing them through their guts.
Small pieces of leaf (5 × 5 mm2) were dehydrated in ethanol series and sonicated to remove unwanted parts from the surfaces.
The modified procedure asked the student to cut 5 mm × 5 mm pieces of leaf, which were then fixed in paraformaldehyde glutaraldehyde solution.
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After 2 months of growth, a small piece of leaf tissue (1 × 1 mm) was excised for early PCR analysis.
The DNA was extracted from a piece of leaf blade of the parental cultivars and F2 individuals.
Genomic DNA was isolated from a small piece of leaf tissue about 2 × 1 cm taken from putative transgenic lines using the cetyltrimethylammonium bromide (CTAB) method (Lee et al. 2015).
A piece of leaf, 5 cm in length, was cut from the tip of each leaf blade, placed in a microtube containing extraction buffer [200 mM Tris-HCl (pH 8.0), 250 mM NaCl, 25 mM EDTA, 0.5% (w/v) sodium dodecyl sulfate], and homogenized with a pestle.
The sample is then ready for PCR amplification. 1. Collect a small piece of leaf in a 1.5-mL tube.
After 12 days at ambient temperature leaf tissue was collected by tearing a piece of leaf (equivalent to size of approximately 1/2″ × 1/2″) from each plant and placing it in an individual well of a 96-well plate.
Three types of patches were set up: (1) 16 aphids with one aphid/leaf ('16ap'), (2) 8 aphids and 8 exuviae equally distributed to form a latin square ('8ap + 8ex') and (3) 8 aphids distributed every other piece of leaf ('8ap').
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CEO of Professional Science Editing for Scientists @ prosciediting.com