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Protein concentrations were determined with SDS-PAGE, and then the gel pieces containing the recombinant proteins were extracted and injected directly into health rabbits.
The gel pieces containing the protein spots were destained by rinsing three times with 25 mM ammonium bicarbonate in 50% (v/v) acetonitrile for 45 min each.
Gel pieces containing the zygotes and proembryos after hybridization were incubated in a 1 2000 Anti-DIG-Antibody (Roche), and transcripts were detected colorimetrically by the DIG nucleic acid detection kit (Roche).
Briefly, ventral midbrain was isolated and the pieces containing the SN were enzymatically dissociated with Papain (SIGMA) under continuous oxygenation (5% CO2 and 95% O2 gas mixture) with slow stirring at 35°.
For immunoblotting experiments, the gel pieces containing the region of interest were incubated in transfer buffer (25 mM Tris- HCl, pH 8,0, 192 mM glycine and 0,04% SDS) for 15 minutes.
Gel pieces containing the proteins of interest were excised and subjected to in-gel trypsin digestion, followed by liquid chromatography tandem mass spectrometry (LC MS/MS) analysis (performed by APRO Life Science Institute, Inc., Tokushima, Japan).
Similar(54)
It is a traditional show in that most of the pieces contain the human figure, but the emotional intensity that runs throughout is peculiar to this art, which spans the period from 1880 to 1933, when Hitler came to power in Germany.
Three pieces contain the letters ECT, which, according to Brynnan K. Light-Lewis, who in 2012 wrote a thesis on Deeds' life and work, may refer to electroconvulsive therapy.
When the piece containing the defect is determined, then subdivisions within that part may be performed and so forth until the bug is cornered in such a small area that it can no longer hide.
The volume of the gel piece containing the P-labeled PCR product was, in average, 30-50 µl.
We opened the remaining piece containing the corpus and antrum along the lesser curvature and spread it out in the form of a trapeze.
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