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In a rather too elegant wall piece, "Leaf Radiance," a large spray of gold leaves, each lettered with "radiance," is held by a wan hand.
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After 2 months of growth, a small piece of leaf tissue (1 × 1 mm) was excised for early PCR analysis.
The DNA was extracted from a piece of leaf blade of the parental cultivars and F2 individuals.
Genomic DNA was isolated from a small piece of leaf tissue about 2 × 1 cm taken from putative transgenic lines using the cetyltrimethylammonium bromide (CTAB) method (Lee et al. 2015).
The sample is then ready for PCR amplification. 1. Collect a small piece of leaf in a 1.5-mL tube.
Three types of patches were set up: (1) 16 aphids with one aphid/leaf ('16ap'), (2) 8 aphids and 8 exuviae equally distributed to form a latin square ('8ap + 8ex') and (3) 8 aphids distributed every other piece of leaf ('8ap').
B. Genomic DNA isolation for individual leaves for fine mapping (see Methods and Results section: "Fine mapping," step 2). 1. Collect a small piece of leaf in a 1.5-mL tube.
For embryogenic calli, two sample types were taken: one sample was just composed of a fragment of the callus, and the other from a fragment of callus with a piece of leaf from a control plant.
For some gilding operations the gilder uses a cushion to hold his pieces of leaf.
Larger soil invertebrate animals perform the function of biting off (shredding) pieces of leaf litter in forest soils and passing them through their guts.
Small pieces of leaf (5 × 5 mm2) were dehydrated in ethanol series and sonicated to remove unwanted parts from the surfaces.
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CEO of Professional Science Editing for Scientists @ prosciediting.com