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When the piece containing the defect is determined, then subdivisions within that part may be performed and so forth until the bug is cornered in such a small area that it can no longer hide.
Fragments ranging from 140 to 190 bp were excised; the gel piece containing the selected DNA fragments was shredded and dispersed into 400 µl of Platinum PCR Supermix with 750 nM of each P1 and P2 PCR primer, 2.5 U of Pfu (Stratagene) and 5 U Taq (Bioline).
The volume of the gel piece containing the P-labeled PCR product was, in average, 30-50 µl.
We opened the remaining piece containing the corpus and antrum along the lesser curvature and spread it out in the form of a trapeze.
Spinal cords were cleaned from their meninges before being divided into four parts corresponding to segments C1 C2, segments C3 C8, segments L1 L5, and a 4-mm piece containing the site of injury.
Similar(55)
"You see, in a hologram, a true hologram, every piece contains the whole," Simon tells Sara, the journalist, just before taking her to bed.
Topped off by a picture of Forrest Gump, the piece contained the following passage: "And 'fickle' is a nice way of describing the voters of 2012, who appear to be wandering confused and Forrest Gump-like through the experience of a presidential campaign.
"This piece contains the kind of inaccuracies and misrepresentations you would expect from something sourced "anonymously", and we'll leave it at that".
The piece contains the following sentence, among many other doozies: "To end cronyism we must end government's ability to dole out favors and rig the market".
The ventral portion of the diencephalic piece contains the ventral thalamus and hypothalamus and was used as the "hypothalamus" explant.
Protein concentrations were determined with SDS-PAGE, and then the gel pieces containing the recombinant proteins were extracted and injected directly into health rabbits.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com