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Briefly, pictures were aligned manually using Adobe Photoshop to generate the image stack needed to perform the 3D reconstruction of the brain.
Pictures were aligned to a movie with a frequency of 25 pictures per second using the free software JPGVideo (NDW Ltd., Nottingham, England).
FIB and SEM pictures were aligned using the FIB-SEM intersection (setting the eucentric position of the stage), and the magnification was set between 2500× and 3000×.
Pictures were aligned, piled and registered using the Fiji plug-in of NIH ImageJ "Register Virtual Stack Slices" (http://fiji.sc/wiki/index.php/Register_Virtual_Stack_Slices).sc/wiki/index.php/Register_Virtual_Stack_Slices
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You should definitely take notes and pictures of how the parts were aligned when you take them out, so that you can get them back in correctly.
The genome nucleotide sequences of N. galegae HAMBI 1141 and HAMBI 540T were aligned to provide an overall picture of the synteny between the two genomes.
Model structures were aligned and analyzed by using the DeepView Swiss-PdbViewer v4.0.1 program [54], and structural pictures were created with the VMD 1.8.7 program [55].
The Yankees' dominoes were aligned.
The public and private interests were aligned.
The sequences were aligned manually.
Sequences were aligned in Vector NTI.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com