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Dr. Waterman and her colleagues at the University of North Carolina then took pictures of cells every few seconds to track the glowing molecules.
Now they are being used to take three-dimensional pictures of individual cells, to map the distribution of trace elements inside cells and to work out hitherto intractable molecular structures.The pictures of cells are made as Carolyn Larabell of the Lawrence Berkeley National Laboratory, in California, described using a technique called soft X-ray tomography.
All pictures of cells with a specific fluorophore were acquired using the same exposure time: 100 ms for DAPI and 400 ms for GFP.
For quantitative analysis of the microscopy data, pictures of cells with DAPI-stained nuclei, and with HOG1 GFP expression, were overlapped.
Pictures of cells were taken with a Nikon camera after fixing cells with 2% glutaraldehyde in PBS.
Pictures of cells along the wound line were taken by phase-contrast microscope.
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I got bored of posting pictures of cell towers and maps every time a regional network story popped up.
These results are consistent with the number of cells observed in the microscopic pictures of cell culture plates and the direct cell counting from trypan blue exclusion assay.
The pictures of cell cultures, upon γ-irradiation in the presence or absence of nicotine, were taken by the phase contrast photography (Fig. 1b).
Phase contrast pictures of cell lines were taken with an inverted Olympus IX-70 microscope connected to an Olympus DP70 digital camera.
The pictures of cell colonies were taken by a digital camera.
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